Detect coliform organisms in water, wastewater, foods, and dairy products using Thermo Scientific™ Remel™ Brilliant Green Bile Broth 2% (Dehydrated)
Researchers found that the combination of bile (Oxygall) and brilliant green inhibited growth of organisms other than coliforms 1
This medium met the specifications of the Association of Analytical Chemists (AOAC), the International Dairy Federation (IDF), and the American Public Health Association (APHA) and is used in the confirmation of presumptive tests for coliforms 2-6
It is also recommended by the Environmental Protection Agency (EPA)
This medium contains gelatin peptone which provides nutrients necessary for growth
Lactose provides a source of energy
Gas production in the Durham tube helps detect fermentation
In this medium, coliforms replicate, ferment and become resistant to these inhibitors
Easy-to-read - Coliform bacteria are easy to recognize by rapid production of gas through lactose fermentation
Selects for coliform organisms - Bile salts and Brilliant green in the medium inhibit nontarget Gram-positive and Gram-negative organisms
The medium is further optimized to prevent the growth of anaerobic lactose fermenters without coliforms being affected