Thermo Scientific™ Remel™ Cetrimide Selective Agar (Dehydrated)

Description

• Selectively isolate and presumptively identify Psuedomonas aeruginosa and other non-fermentative, gram-negative bacilli using Thermo Scientific™ Remel™ Cetrimide Selective Agar (Dehydrated)
• King et al
• developed a medium called Tech Agar which was used to enhance pyocyanin production by Psuedomonas 1
• In 1955, Lowbury described the use of cetrimide in a selective medium for P
• aeruginosa 2
• This formulation is recommended by the United States Pharmacopeia (USP) and the AOAC International (AOAC) 3,4
• Centrimide Agar has the formula of Tech Agar but is modified by the addition of centrimide
• Selectively isolate and presumptively identify Psuedomonas aeruginosa and other non-fermentative, gram-negative bacilli using Cetrimide Selective Agar (Dehydrated)
• Selective: Centrimide in the medium inhibits bacteria other than P
• aeruginosa by causing nitrogen and to be released from bacterial cells, making the medium selective for P
• aeruginosa
• Easy-to-read: P
• aeruginosa forms fluorescent green colonies which can be easily differentiated from the other non-fluoroscent colonies
• This formulation is recommended by the United States Pharmacopeia (USP) and the AOAC International (AOAC) 3,4
• Centrimide Agar has the formula of Tech Agar but is modified by the addition of centrimide
• This medium contains potassium sulfate and magnesium chloride which stimulate the production of pyocyanin and fluorescein
• Fluorescein production is visualized using an ultraviolet light
• P
• aeruginosa is characterized by production of pyocyanin, a blue-green, water-soluble, non-fluorescent phenazine pigment
• Centrimide (N-acetyl-NNN-trimethyl-ammonium bromide, cetavlon) causes nitrogen and phosphorus to be released from bacterial cells and inhibits bacteria other than P
• aeruginosa .