Thermo Scientific™ Remel™ Pseudomonas P Agar

Description

• Detect and differentiate Pseudomonas aeruginosa from other Pseudomonas spp
• using Thermo Scientific™ Remel™ Pseudomonas P Agar (Dehydrated)
• In 1954, King et al
• developed two media formulations (Medium A and Medium B) to enhance pigment production in pseudomonads 1
• Earlier work had demonstrated that the production of pyocyanin and fluorescein is influenced by amino acids, inorganic ions, minerals, and peptones
• King determined that the composition of the peptone in the medium affected the production of these pigments
• Pseudomonas aeruginosa is the only species of Pseudomonas known to produce and excrete the pigment pyocyanin
• Pseudomonas P Agar was a medium developed by King, Ward, and Raney to improve pyocyanin production by Pseudomonas aeruginosa 1
• The absence of minimal concentrations of components which may have a detrimental effect influences pigment production
• A combination of magnesium chloride and potassium sulfate was found to be most effective in stimulating pyocyanin production 2
• The medium contains gelatin and meat peptones that provide nutrients necessary for bacterial growth
• These peptones contain less than 0.1% phosphorus, which has been found to increase pyocyanin production
• The total concentration of magnesium chloride and potassium sulfate does not exceed 2%, which stimulates pyocyanin production
• Addition of glycerol to the prepared medium acts as an energy source.