Thermo Scientific™ Mannitol Salt Agar (Dehydrated)

Description

• Selectively and differentially isolate staphylococci using Thermo Scientific™ Remel™ Mannitol Salt Agar (Dehydrated)
• This phenol red mannitol agar contains 7.5% sodium chloride as a selective agent as reported by Koch in 1942 1
• Most strains of coagulase-positive staphylococci grow on this agar, producing yellow zones because of mannitol fermentation, whereas coagulase-negative staphylococci strains produce small colonies with no color change in the surrounding medium
• This agar is formulated in conformance with harmonized United States Pharmacopeia (USP)/ European Pharmacopeia (EP) guidelines 2,3
• Mannitol Salt Agar is a selective and differential solid media for isolating staphylococci by their ability to ferment mannitol
• Conforms to USP and EP : Formulated in conformance with harmonized United States Pharmacopeia (USP)/ European Pharmacopeia (EP) guidelines 2,,3
• Easy to differentiate : Differentiates between coagulase-positive and coagulase-negative staphylococci on the basis of mannitol fermentation Selective : Sodium chloride at a concentration of 7.5% inhibits many bacteria other than staphylococci
• This medium contains casein and meat peptones that supply nitrogen, amino acids, and peptides necessary for bacterial growth
• Sodium chloride in the concentration of 7.5% is a selective agent that inhibits many bacteria other than staphylococci
• Phenol red is the pH indicator which causes a color change in the medium from red-orange to yellow when acid is produced
• Colonies of staphylococci that ferment mannitol will be surrounded by a yellow zone, while those that do not ferment mannitol will have no color change (red)
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand