MP Biomedicals™ SC-Ura (Powder)

Description

• The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine)
• Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component
• SC-Ura medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs
• To use, add 1.9g of SC-Ura to each liter of DOB or DOBA and autoclave at 121°C for 15 minutes for complete synthetic defined medium for S.cerevisiae.