Selectively and differentially isolate enteric gram-negative bacilli using Thermo Scientific™ Remel™ MacConkey Broth
MacConkey Broth is a modification of the original bile salt broth first described by MacConkey in 1900, which contained litmus as an indicator and sodium taurocholate to inhibit gram-positive organisms 1
In further testing, MacConkey recommended replacing litmus with neutral red 2
In 1953, Childs and Allen replaced neutral red with bromcresol purple, a less inhibitory indicator 3
Selectively and differentially isolate enteric gram-negative bacilli using MacConkey Broth
This medium is used for presumptive identification of coliforms
Coliforms are organisms that are present in the environment and in the feces of all warm-blooded animals and humans
Their presence in drinking water indicates that disease-causing organisms (pathogens) could be in the water system
Selective: Oxgall is a selective agent which inhibits gram-positive organisms
Ease of differentiation: Lactose and bromcresol purple indicator enable the differentiation of lactose fermenting gram-negative bacilli
Conforms to USP and EP : It is formulated and tested in conformance with harmonized United States Pharmacopeia (USP)/ European Pharmacopeia (EP) guidelines 4
This medium contains gelatin peptone that provides amino acids and essential nutrients for bacterial growth
Lactose is a carbon source for energy
Oxgall is a selective agent which inhibits gram-positive organisms
Lactose and bromcresol purple indicator enable the differentiation of lactose fermenting gram-negative bacilli
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