Thermo Scientific™ Tergitol 7 Agar w/TTC

Description

• Selectively isolate and differentiate coliform organisms with Thermo Scientific™ Remel™ Tergitol 7 Agar w/TTC
• The use of tergitol (sodium heptadecyl sulfate) as a selective agent was first proposed by Pollard in 1946 1
• Tergitol 7 Agar was later developed by Chapman for selective isolation and increased recovery of the coliform organisms 2,3
• Later Tergitol 7 Agar was modified by addition of triphenyltetrazolium chloride (TTC) to facilitate early recognition of Escherichia coli and Enterobacter aerogenes within 6-10 hours of incubation
• Tergitol 7 Agar w/TTC is recommended for the selective isolation and differentiation of the coliform groups
• Microorganisms capable of fermenting lactose produce yellow colonies on Tergitol 7 agar due to reaction of brom thymol blue indicator
• Non-lactose fermenters produce blue to green or purple colonies
• TTC incorporated into Tergitol 7 Agar is a rapid indicator of bacterial growth
• Some coliforms reduce TTC to insoluble formazan resulting in red colonies with blue zones that appear as purple
• E
• coli and E
• aerogenes do not reduce TTC and form yellow colonies with yellow zones
• Ready-to-use – Convenience of prepared media
• Selective – Tergitol is a selective agent, which inhibits the growth of the gram-positive organisms and Proteus Easy-to-interpret – E
• coli and E
• aerogenes do not reduce TTC and form yellow colonies with yellow zones
• Tergitol 7 Agar w/TTC contains yeast extract, casein and meat peptone that provide nitrogen, amino acids and peptides necessary for bacterial growth
• Lactose serves as the source of fermentable carbohydrate
• Tergitol-7 acts as a selective agent that inhibits gram positive organisms and minimises the swarming of Proteus, allowing superior recovery of coliforms
• Brom thymol blue is the pH indicator
• Tri-phenyltetrazolium chloride (TTC) allows earlier recognition and identification of Escherichia coli and Enterobacter aerogenes
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.