Demonstrate motility in microorganisms with Thermo Scientific™ Remel™ Motility Test Medium w/TTC Indicator
Motility Test Medium w/TTC Indicator is a modification of the formulation of Tittsler and Sandholzer for detection of motility in enteric gram-negative bacilli 1
When results of motility determinations in hanging-drop preparations and semi-solid agar were compared, they observed perfect correlation 2
Kelly and Fulton recommended the addition of a tetrazolium salt to enhance the macroscopic appearance of motile organisms in motility media 3
Motility Test Medium w/TTC Indicator is a semisolid media that is used to demonstrate motility in microorganisms
Agar provides a semisolid medium which allows macroscopic motility interpretations
TTC (2,3,5-triphenyltetrazolium chloride) allows visual enhancement of bacterial growth
Tetrazolium salt is colorless, but as the organism grows the dye is incorporated into bacterial cells and is reduced to an insoluble red pigment, formazan
This red color forms only in the area where bacteria is growing
Ready- to-use: Convenience of prepared media Easy to interpret: Observable color change When TTC is added to the medium, red color develops in the area of bacterial growth
Motility Test Medium w/TTC Indicator contains gelatin peptone and beef extract that supply vitamins and nitrogenous compounds essential for bacterial growth
Sodium chloride provide essential electrolytes and maintains osmotic equilibrium
TTC Indicator aids visual enhancement of bacterial growth
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.