Gibco™ M9 Minimal Salts (2X)

Description

• M9 Minimal Salts media is a commonly used basic and chemically defined bacterial growth media for cultivation of E
• coli strains
• It provides only the necessary nutrients (carbon, nitrogen, phosphorus, sulfur, and metal sources) required for bacterial growth
• Our Gibco M9 Minimal Salts (2X) is a ready-to-use concentrated base formulation that can be supplemented with various carbon sources and amino acids to make a complete M9 medium
• This medium is preferred in microscopy of E
• coli experiments as it has a low autofluorescence and absorbance properties
• Growth rates decrease at lower or higher osmolarity, and M9 medium has a lower osmolarity (∼0.25 Osm/L) than E
• coli needs to optimally grow (∼0.3 Osm/L)
• This allows for tight control over bacterial growth
• M9 Minimal Salts (2X) formulation Formulation per liter: 25.6 g Sodium phosphate (dibasic) heptahydrate 6 g Monopotassium phosphate 1 g Sodium chloride 2 g Ammonium chloride • A carbon source needs to be added to required concentration • Additional components might be needed for E
• coli strains (e.g
• thiamine for DH5α, leucine for DH10B) Primary applications • Recombinant protein induction in E
• coli in extended growth phase (improved protein expression) • Microscopy or fluorescence measurements experiments using bacteria • Metabolic activity studies in bacteria with controlled chemical media composition Gibco media bottles Each media bottle features an angled opening for simplified pipetting
• The 1 L Gibco media bottle uses 39% less source material than comparable media bottles on the market
• Both the cap and bottle can be recycled as they are made from highly recyclable high-density polyethylene (HDPE) and polyethylene terephthalate (PETE)
• Find the media format that fits your needs We offer other ready-to-use media formulations, such as LB Broth (Cat
• No 10855001), Terrific Broth (Cat
• No
• A1374301) or S.O.C recovery medium (Cat
• No
• 15544034).