by satellite phenomena with Thermo Scientific™ Remel™ Non-supplemented Buffered CYE Agar (Buffered charcoal yeast extract-BCYE)
BCYE Agar was developed by adding ACES buffer and α-ketoglutarate, which stabilized the pH and improved the isolation of Legionella pneumophila from contaminated clinical and environmental specimens 1,2
Cysteine is essential for the growth of Legionella spp
Isolates that grow on BCYE Agar containing L-cysteine but fail to grow on Non-supplemented BCYE Agar may be presumptively identified as Legionella spp 3–4
Buffered CYE (BCYE) Agar Nonsupplemented is a solid medium recommended for the presumptive identification of Legionella spp 4
BCYE Agar Nonsupplemented contains charcoal and yeast extract to enhance the growth of Legionella
Charcoal also serves to absorb toxic metabolic products and modify the surface tension of the medium
ACES Buffer maintains proper pH and α-ketoglutarate is added to stimulate growth
Agar is a solidifying agent
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.