Detect the deoxyribonuclease (DNase) activity in microorganisms with Thermo Scientific™ Remel™ DNase Test Agar
In 1957, Jeffries et al
developed a medium for demonstrating deoxyribonuclease (DNase) activity of microorganisms possessing this extracellular enzyme
1 Such microorganisms depolymerize DNA in the medium resulting in a clear zone around the colonies when the plate is flooded with 1N hydrochloric acid (HCl)
DNase Test Agar has been recommended for identification of a variety of bacteria including staphylococci, enteric gram-negative bacilli, and pseudomonads
2-5 This is a solid medium used to detect the deoxyribonuclease (DNase) activity in microorganisms
Easy- to-interpret: Positive test – after 1N HCl is added, a clear zone is formed around the growth line and the rest of the plate remain opaque
Negative test – after 1N HCl is added, no clear zone is formed around the growth line and the entire plate is opaque
Ready- to-use: convenience of prepared media
DNase Test Agar contain casein and soy peptone that supply nitrogen, amino acids and peptides necessary for bacterial growth
Sodium chloride provide essential electrolytes which maintain osmotic equilibrium
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.