Selectively recover Salmonella species other than Salmonella enterica serovar Typhi with Thermo Scientific™ Remel™ XLT4 Agar Monoplate
XLT4 (Xylose-Lysine-Tergitol 4) Agar was developed by Miller and Tate to selectively screen samples contaminated with enteric organisms for the presence of Salmonella 1
They found that XLT4 Agar completely inhibits competing enteric organisms
Mallinson et al
reported XLT4 Agar demonstrated significant improvements over traditionally used differential media for the detection of Salmonella from raw red and white meat samples and poultry litter 2
Salmonella is a rod-shaped, gram-negative facultative anaerobe that belongs to the family Enterobacteriaceae and is one of the most commonly isolated foodborne pathogens found predominantly in poultry, eggs and dairy products
Numerous Salmonella serotypes have been identified, majority of which belong to Salmonella enterica subsp
enterica , and can cause serious, at times life-threatening, infections in humans
XLT4 Agar Monoplate is a solid medium that is used for selective and differential recovery of non-typhi Salmonella species from clinical specimens
Easy-to-use – Convenience of prepared media
Selective – Selective agent Tergitol 4 reduces the growth of non-salmonellae enteric gram-negative bacilli
Easy-to-differentiate – H 2 S-positive organisms like salmonellae produce black-yellow colonies
XLT4 Agar Monoplate contains meat peptone and yeast extract that supply nitrogenous compounds, amino acids and vitamins necessary for bacterial growth
Xylose is the carbohydrate source
Lysine helps to isolate salmonellae based on their ability to decarboxylate lysine and produce an alkaline pH
Lactose and sucrose are added in excess to prevent colonies of lysine-positive coliforms from reverting to alkaline conditions
Sodium thiosulfate is a sulfur source and ferric ammonium citrate is an indicator of H 2 S-production
Tergitol 4 acts as a selective agent and enhances the specificity and sensitivity of Salmonella detection and reduces the growth of non-salmonellae enteric gram-negative bacilli
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