Thermo Scientific™ McClung Toabe Agar

Description

• Selectively isolate and differentiate Clostridium spp
• using Thermo Scientific™ Remel™ McClung-Toabe Agar
• In 1947, McClung and Toabe developed this differential plating medium to detect lecithinase and lipase production by Clostridium spp 1
• This agar was further modified by Dowell and Hawkins by adding neomycin to selectively isolate and differentiate certain obligately anaerobic bacteria such as Clostridium perfringens 2
• Use McClung-Toabe Agar for selectively isolate and differentiate Clostridium spp
• Selective – Neomycin inhibits many gram-positive and gram-negative organisms Easy-to-interpret – Based on lecithinase and lipase production by Clostridium spp 1 Ready-to-use – Convenience of prepared media Clostridium spp
• produce the enzymes lecithinase and lipase
• Egg yolk suspension (50%) present in the medium serves as the substrate to detect lecithinase and lipase activity 3
• Lecithinase degrades lecithin and produces an insoluble, opaque precipitate in the medium surrounding growth 4
• Lipase breaks down free fats in the egg yolk forming in an iridescent sheen on the colony surface
• This medium contains gelatin peptone that supplies amino acids and other nitrogenous compounds necessary for the growth of anaerobic bacteria
• Dextrose serves as an energy source
• Egg yolk serves as a substrate for detection of lecithinase and lipase activity 3 and also reduces the toxic effect of organic peroxides which may accumulate in the medium
• Neomycin acts as the selective agent which inhibits many gram-positive and gram-negative organisms
• Agar is the solidifying agent.