Selectively isolate Haemophilus species from respiratory specimens with Thermo Scientific™ Remel™ Haemophilus Isolation Agar w/bacitracin and horse blood
Haemophilus influenzae, a fastidious gram-negative bacillus, is a common cause of serious diseases of the upper and lower respiratory tract 1
Haemophilus Isolation Agar w/bacitracin and horse blood is a solid media recommended for use in qualitative procedures for selective isolation of Haemophilus spp
from respiratory specimens
Selective : Kanamycin and vancomycin antibiotics inhibit gram-positive organisms and facultative anaerobic bacteria
Neomycin stimulates the growth of gram-negative anaerobes, especially Bacteriodes spp
Colistin and nalidixic inhibit the growth of most gram-negative bacilli
Enriched medium: The presence of intact erythrocytes in horse blood enables differentiation of hemolytic and nonhemolytic Haemophilus spp
Horse blood, unlike sheep blood, does not contain V factor-inactivating enzymes
Ready-to-use : Convenience of prepared media
Beef heart Infusion and casein peptone supply the nutritional requirements for all but the fastidious bacteria
Horse sheep supplies both the X and V factors, which are growth requirements for certain organisms, such as Haemophilus influenzae
Horse blood also serves to distinguish the hemolytic species of Haemophilus from non-hemolytic Haemophilus species
Bacitracin inhibits normal flora found in respiratory cultures, particularly Neisseria spp
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