Thermo Scientific™ Mannitol Salt Agar with Oxacillin (4μg/mL)

Description

• Selectively and differentially isolate methicillin-resistant staphylococci from clinical specimens using Thermo Scientific™ Remel™ Mannitol Salt Agar with Oxacillin (4μg/mL)
• This agar contains 7.5% sodium chloride as a selective agent as reported by Koch in 1942 1
• Coagulase-positive staphylococci strains grow on this agar, producing yellow zones because of mannitol fermentation whereas coagulase-negative staphylococci strains produce tiny colonies with no color change
• Staphylococci are widespread in nature, although they are mainly found on the skin, skin glands and mucous membranes of mammals and birds
• The coagulase-positive species, i.e
• Staphylococcus aureus, is well documented as a human opportunistic pathogen
• The ability to clot plasma continues to be the most widely used and accepted criterion for the identification of pathogenic staphylococci associated with acute infections 2
• One of the pathogenic strains is methicillin-resistant strain
• Methicillin resistance is associated with production of a unique penicillin-binding protein (PBP), 2a, which is bound and inactivated only at high concentrations of beta-lactam antibiotics
• The heterogeneous expression of methicillin resistance by staphylococci poses problems in the detection of resistant strains 3
• Use Mannitol Salt Agar with Oxacillin for selective and differential isolation of methicillin-resistant staphylococci
• Easy-to-differentiate : Differentiates between coagulase-positive and coagulase-negative staphylococci
• Selective : Sodium chloride in the concentration of 7.5% inhibits many bacteria other than staphylococci
• Ready-to-use: Convenience of prepared media
• This medium contains casein and meat peptones that supply nitrogen, amino acids, and peptides necessary for bacterial growth
• Beef extract is a source of essential nutrients, carbon and sulfur for microbial growth
• Phenol red is the pH indicator which causes a color change in the medium from red-orange to yellow when acid is produced
• Colonies of Staphylococci that ferment mannitol will be surrounded by a yellow zone, while those that do not ferment mannitol will have no color change
• Oxacillin (4μg/ml) is added to inhibit the growth of non-MRSA staphylococci and other susceptible organisms
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.