Thermo Scientific™ MRS Agar

Description

• Isolate and enumerate Lactobacillus species and gram-positive cocci from clinical and non-clinical samples using Thermo Scientific™ Remel™ MRS Agar
• In 1960, De Man, Rogosa and Sharpe modified a medium earlier formulated by Rogosa et al 1 by eliminating tomato juice and created a medium which supported the growth of slower growing lactobacilli 2
• Recently MRS Agar has been used in the clinical laboratory to differentiate certain strains of gram-positive cocci and lactobacilli from clinical samples 3-6
• ‘Lactic acid bacteria’ are Gram-positive, catalase and oxidase negative and fastidious in their nutritional requirements
• Growth is enhanced considerably by microaerobic conditions
• Generally, the ‘lactic acid bacteria’ show delayed growth and smaller colony size than other microorganisms
• They may be overgrown in non-selective media, especially if incubation is required for 2-4 days
• Can be used to differentiate certain strains of gram-positive cocci and lactobacilli
• Supports the growth of slower growing lactobacilli
• Selective : Ammonium citrate and sodium acetate inhibit the growth of commensal microbial flora, gram-negative bacilli and fungi, and improve the growth of lactobacilli
• Ready-to-use : Convenience of prepared media
• This medium contains gelatin peptone and beef extract that supply essential nutrients and amino acids necessary for bacterial growth
• Yeast extract provides vitamin B complex and dextrose is the fermentable carbohydrate and energy source
• Polysorbate 80 supplies fatty acids required for the metabolism of lactobacilli
• Magnesium sulphate and manganese sulphate provide essential ions for multiplication of lactobacilli
• Phosphates provide good buffering action in the media
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.