Thermo Scientific™ m Enterococcus Agar

Description

• Selectively isolate and enumerate enterococci by membrane filtration or by direct plating using Thermo Scientific™ Remel™ m-Enterococcus Agar
• In 1957, Slanetz and Bartley reported that m-Enterococcus Agar was superior to other media for detecting and enumerating fecal streptococci by the membrane filtration technique 1
• Saraswat et al
• used m-Enterococcus Agar to select enterococci in dried foods, including non-fat dry milk 2
• m-Enterococcus Agar is recommended in Standard Methods for the Examination of Water and Wastewater and Standard Methods for the Examination of Dairy Products
• The Enterococcus count is used as an indicator of sanitary quality in water and dairy products 3,4
• Use m-Enterococcus Agar for selective isolation and enumeration of enterococci by membrane filtration or by direct plating
• Recommended in Standard Methods for the Examination of Water and Wastewater and Standard Methods for the Examination of Dairy Products Selective – Sodium azide inhibits gram-negative organisms Easy-to-interpret – Enterococci form pink to red colonies of 0.5–3 mm in diameter Ready-to-use – Convenience of prepared media
• The medium contains casein and soy peptones that provide nitrogenous compounds, amino acids, and peptides necessary for bacterial growth
• Yeast extract supplies B-complex vitamins which are essential for bacterial metabolism
• Dextrose provides a ready source of energy
• Dipotassium phosphate acts as a buffer
• Sodium azide is a selective agent that inhibits gram-negative organisms
• Triphenyltetrazolium chloride (TTC) is the dye that serves as an indicator of bacterial growth and agar is the solidifying agent
• TTC gets reduced to soluble formazan inside the bacterial cell resulting in the production of red colonies.