Thermo Scientific™ Vogel Johnson Agar

Description

• Detect coagulase-positive, mannitol fermenting strains of Staphylococcus aureus from contaminated foods and clinical specimens using Thermo Scientific™ Remel™ Vogel-Johnson Agar
• In 1955, Zebovitz et al
• developed Tellurite-Glycine Agar as a selective medium for the detection of coagulase-positive staphylococci 1
• Vogel and Johnson increased the mannitol content and added phenol red as a pH indicator to create Vogel-Johnson Agar 2
• This agar is recommended by Association of Official Analytical Chemists (AOAC) for food bacteriology 3,4
• Staphylococcus is a common cause of food poisoning due to production of enterotoxins
• Staphylococcus aureus is a gram-positive bacteria that causes a wide variety of clinical diseases
• Infections caused by this pathogen are common both in community-acquired and hospital-acquired settings
• Vogel-Johnson Agar, by selectively identifying coagulase-positive and mannitol-fermenting strains, permits the early detection of Staphylococcus aureus from heavily contaminated foods and clinical specimens
• Ready to use – Convenience of a prepared media Selective – Potassium tellurite, lithium chloride and glycine act as selective agents which inhibit non-staphylococcal organisms, including many gram-negative and gram-positive bacteria
• Easy to interpret – Coagulase-positive staphylococci ferment mannitol to form yellow zones around the colonies
• S
• aureus reduces potassium tellurite to metallic tellurium resulting in black colonies
• Recommended by AOAC – For isolation of staphylococci from food sources 3,4
• This medium contains casein peptone that provides essential amino acids, peptides, and nitrogen essential for the growth of bacteria
• Yeast extract supplies essential B-complex vitamins
• Potassium tellurite, lithium chloride and glycine are selective agents which inhibit non-staphylococcal organisms, including many gram-negative and gram-positive bacteria
• Phenol red is the pH indicator and agar is the solidifying agent
• The fermentation of mannitol by coagulase-positive staphylococci is indicated by development of yellow zones surrounding the colonies
• This is caused by a pH change, resulting in the phenol red indicator turning from red to yellow
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.