Thermo Scientific™ TOC Agar (Polysorbate 80, Oxgall, Caffeic Acid)

Description

• Presumptively identify Candida albicans and Cryptococcus neoformans by germ tube formation, chlamydospore production and phenol oxidase activity using Thermo Scientific™ Remel™ TOC Agar (Polysorbate 80, Oxgall, Caffeic Acid)
• Two media were developed for the sequential development of germ tubes and chlamydospores by C
• albicans within 24-48 hours 1,2 , containing a combination of rice infusion, oxygall, polysorbate 80, 0.1% glucose and 2% New Zealand Agar
• Later a culture medium was developed to aid in the identification of both C
• neoformans and Candida spp
• 3 which became known as TOC Agar
• TOC Agar is a differential medium used for the presumptive identification and differentiation of Candida albicans and Cryptococcus neoformans
• It contains polysorbate 80 and oxgall which increase the rate at which germ tubes and chlamydospores are formed
• Caffeic acid serves as a substrate for detection of phenol oxidase produced by C
• neoformans
• This enzymatic reaction produces melanin which is absorbed by the yeast cell wall resulting in brown-pigmented colonies
• Ready to use – Convenience of prepared medium Easy to interpret – In germ tube production a short hyphal (filamentous) extension arises laterally from a yeast cell, with no constriction at the point of origin
• In chlamydospore production clusters of round blastoconidia at the septa and large, thick walled terminal chlamydosopres are seen and in pigment production growth tan to brown-pigmented colonies are observed Not all products are available for sale in all territories
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.