Thermo Scientific™ Middlebrook 7H11 Agar/Middlebrook 7H11 Agar Biplate

Description

• Isolate and cultivate Mycobacterium species using Thermo Scientific™ Remel™ Middlebrook 7H11 Agar/Middlebrook 7H11 Agar Biplate
• Dubos and Middlebrook developed a medium containing oleic acid and albumin to enhance the growth of tubercle bacilli and protect them against a variety of toxic agents 1
• Middlebrook and Cohn improved this medium and developed 7H10 Agar that allowed faster, more luxuriant growth of Mycobacteria 2
• Cohn et al
• further supplemented this agar with casein hydrolysate to create 7H11 Agar that stimulates the growth of mycobacteria which otherwise fail to grow 3
• Produce more screening details of pathogenic organisms while reducing storage capacity issues in the incubator with a bi-plate that includes widely used microbiological medium for the detection of Mycobacterium species
• Selective media – Malachite green dye inhibits contaminating bacteria other than mycobacteria Ready-to-use – Convenience of prepared media Space saving– Bi-plate format Increased throughput – Fewer plates to process per sample allows greater throughput in automated specimen processors
• Middlebrook 7H11 Agar is a solid medium that is used to isolate and cultivate Mycobacterium secies from specimens that contain mixed flora
• Middlebrook 7H11 Agar contains casein hydrolysate that stimulates growth of Mycobacterium tuberculosis and inorganic salts essential for the growth of mycobacteria
• Glycerol is the energy source
• Sodium citrate provides inorganic cations in solution
• Supplemental OADC Enrichment contains sodium chloride that maintains osmotic equilibrium abd dextrose as an energy source
• Catalase destoys toxic peroxides that may be present in the medium, oleic acid is a fatty acid that is utilized in the mechanism of mycobacteria, and albumin protects tubercle bacilli against toxic agents.