Thermo Scientific™ Middlebrook 7H11 Agar/Mitchison 7H11 Selective Biplate

Description

• Isolate and cultivate Mycobacterium species from specimens that contain mixed flora with Thermo Scientific™ Remel™ Middlebrook 7H11 Agar/Mitchison 7H11 Selective Biplates
• Middlebrook 7H10 Agar is a solid medium that allows faster and luxurious growth of Mycobacteria 1 and protects the organisms against toxic agents 2
• The Middlebrook 7H11 Agar, developed by Cohn et al., contains casein hydrolysate that stimulates growth of drug-resistant strains of M
• tuberculosis
• Mitchison et al
• modified this formulation by adding antibiotics as selective agents with the 7H11 base 3 to selectively isolate Mycobacteria
• Produce more screening details of pathogenic organisms while reducing storage capacity issues in the incubator with a bi-plate that includes widely used microbiological medium for the detection of Mycobacterium species
• Ready to use: Convenience of prepared media
• Selective : Polymyxin B, carbenicillin, amphotericin B and trimethoprim inhibit growth of contaminating Enterobacteriaceae, yeast and Proteus
• Malachite-green dye partially inhibits bacteria other than mycobacteria
• Space- saving: Biplate format Middlebrook 7H11 Agar/Mitchison 7H11 Selective Biplates contain inorganic salts essential for growth of mycobacteria
• Casein hydrolysate stimulates growth of drug resistant strains of Mycobacterium tuberculosis 4
• Glycerol is the source of carbon and energy
• Sodium citrate is converted to citric acid which holds inorganic cations in solution
• OADC Enrichment is a supplement which contains following additives: sodium chloride to maintain osmotic equilibrium, dextrose that serves as an energy source, catalase to destroy toxic peroxides that may be present in the medium; oleic acid which is required in the metabolism of mycobacteria; and albumin to protect tubercle bacilli against toxic agents
• It also contains selective agents malachite-green dye, polymyxin B, carbenicillin, amphotericin B and trimethoprim
• Polymyxin B and carbenicillin are selective agents active against most Enterobacteriaceae
• Amphotericin B and trimethoprim are active against yeast and Proteus spp., respectively
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• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.