Thermo Scientific™ Nocardia Quad

Description

• Differentiate aerobic actinomycetes by hydrolysis of casein, xanthine, tyrosine, and starch with Thermo Scientific™ Remel™ Nocardia Quad
• A taxonomic scheme to differentiate these genera based on biochemical characteristics was developed by Mishra et al 1
• McGinnis et al
• simplified it to identify the most commonly isolated actinomycetes by including tests for casein, xanthine, and tyrosine degradation 2
• Starch is added to Nutrient Agar to presumptively differentiate Streptomyces from Nocardia based on the inability of Nocardia species to hydrolyze starch 3
• The aerobic actinomycetes as a group are gram-positive, catalase-positive bacteria that may be presumptively identified by staining properties, microscopic morphology, and substrate degradation
• Certain actinomycetes produce a hydrolytic enzyme that degrades the skim milk in Casein Agar, which is demonstrated by clearing of the agar surrounding the area of growth
• Ready to use – Solid media Convenient – Quad plate format Space saving – Read more results from a single sample and produce more diagnostic details while reducing storage capacity issues in the incubator with a quad-plate that includes four separate media formulations Easy to interpret – Casein Agar (I) Positive Test – Clearing of casein agar around and/or under growth Negative Test – Growth with no clearing of the medium Xanthine Agar (II) Positive Test – Clearing of xanthine agar around and/or under growth Negative Test – Growth with no clearing of the medium Tyrosine Agar (III) Positive Test – Clearing of tyrosine agar around and/or under growth Negative Test – Growth with no clearing of the medium Starch Agar (IV) Positive Test – No color development surrounding growth Negative Test - Purple color development surrounding growth Casein agar contains skim milk that acts as a substrate for the hydrolytic enzyme produced by some actinomycetes
• Xanthine and Tyrosine Agars contain beef extract that provides carbohydrates, vitamins, nitrogen compounds, and salts
• The peptones present in these media provide nutrients in the form of amino acids and peptides
• Starch Agar contains potato starch which acts as a substrate to detect amylase production
• This is demonstrated by clearing of the agar surrounding the area of growth, and easily detected upon addition of Gram Iodine or Lugol’s iodine
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.