Thermo Scientific™ GC Agar Base w/1% GCHI

Description

• Perform Antimicrobial disc diffusion susceptibility testing of Neisseria gonorrhoea with Thermo Scientific™ Remel™ GC Agar Base with 1% GCHI
• In 1945, Johnston developed modified chocolate agar that allowed the growth of N
• gonorrhoea in 24 hours rather than 48 hours 1
• Fast growth rate was traced to differences in gel strength of the media tested
• Therefore, GC Agar Base w1% GCHI was developed with reduced agar content
• In 1977, Thornsberry et al
• used GC Agar Base to determine β-lactamase production by N
• gonorrhoeae 2
• Jones et al
• recommended adding a modified GCHI Enrichment into GC Agar Base 3
• GC Agar Base w1% GCHI is a solid medium used for antimicrobial susceptibility testing of Neisseria gonorrhoea using the disc diffusion technique
• The effect of penicillin is inhibited when pathogenic Neisseria produce β-lactamase
• Gonococci with 10U penicillin disc zone diameters ≤ 19 mm are likely to be β-lactamase producing strains 4
• Ready- to-use: Convenience of prepared media
• Easy- to-interpret: Positive test- Penicillin-resistant organism with 10U penicillin disc zone diameter ≤ 19 mm
• Negative test- tiny or individual colonies showing no growth
• Meets CLSI standards
• 4 GC Agar Base w1% GCHI contains casein and meat peptones that supply corn starch to neutralize toxic fatty acids
• The phosphate buffer controls pH changes that can affect organism survival
• The GCHI Enrichment provides NAD, vitamins, amino acids, coenzymes, dextrose, ferric ions and other growth factors needed to cultivate Neisseria species
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.