Thermo Scientific™ Dubos Polysorbate 80 Albumin Broth

Description

• Cultivate Mycobacterium species with Thermo Scientific™ Remel™ Dubos Polysorbate 80 Albumin Broth
• In 1946, Dubos and Davis described a liquid medium in which growth of tubercle bacillus was obtained from dilute inocula in 3 to 5 days 1
• Dubos and Middlebrook 2 then studied the use of this medium to cultivate Mycobacterium tuberculosis
• The original solution was modified in 1950 by Dubos, Fenner and Pierce 3 by adding a strong buffering system and an acid pH which provided dispersed growth from a small inoculum
• This broth is used for subculturing mycobacteria and preparing inoculum for other in vitro tests 4
• Dubos Polysorbate 80 Albumin Broth is a liquid medium recommended for cultivation of Mycobacterium species
• Polysorbate 80 is an oleic acid ester and an in vitro indicator of mycobacterial virulence
• Virulent cultures form cords in media containing oleic acid or its esters, while nonvirulent cultures grow more diffusely
• Ready-to-use: Convenience of prepared media
• Dubos Polysorbate Albumin Broth contains casein peptone and asparagine that supply nitrogen for the growth of mycobacteria
• Disodium and monopotassium phosphates provide the buffering system
• The inorganic salts supply ions essential for metabolism
• Polysorbate 80 (10%) serves as a nutritional source of essential fatty acids and also acts as a dispersing agent for homogenous growth from a small inoculum
• Bovine plasma albumin (serum factor V) enhance the growth of mycobacteria and provides protection against bacteriostatic and bactericidal effect by toxic agents
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.