Thermo Scientific™ Decarboxylase Broth (Moeller) Ornithine

Description

• Differentiate gram-negative enteric bacilli based on the enzymatic ability of an organism to decarboxylate ornithine using Thermo Scientific™ Remel™ Decarboxylase Broth (Moeller) Ornithine
• In 1955, Moeller developed the amino acid decarboxylase media to detect production of lysine and ornithine decarboxylase and arginine dihydrolase 1
• These media are used to differentiate bacterial groups within the Enterobacteriaceae 2
• Ewing, Davis, and Edwards reported Moeller's formulation to be more reliable for differentiating Klebsiella and Enterobacter as compared to other media tested
• 3 The Moeller method is considered the standard or reference method for determining the decarboxylase reactions of the Enterobacteriaceae
• This medium helps differentiate gram negative bacilli by their ability to decarboxylate ornithine
• The ornithine decarboxylase test is especially important for separating members of the Klebsiella - Enterobacter - Serratia group and for identifying species of Proteus
• Recommended as the standard or reference method for determining decarboxylase reactions of Enterobacteriaceae
• Ready to Use: Convenience of prepared media
• Ease of Identification: Visual detection of color change This liquid medium is recommended for use in qualitative procedures
• This basal medium contains meat peptone and beef extract that provide the nitrogenous nutrients required for bacterial growth
• Dextrose acts as a source of energy and fermentable carbohydrate
• Bromcresol purple and cresol red are pH indicators
• Pyridoxal is an enzyme cofactor which enhances decarboxylase activity 4
• Ornithine is an amino acid that is added to the basal medium to detect the production of ornithine decarboxylase
• When an organism ferments dextrose, acids are produced which lower the pH, resulting in a color change from purple to yellow
• If decarboxylation of ornithine occurs in response to the acid pH, the alkaline end-product putrescine (amine) is formed which results in the medium reverting to its original color (purple)
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.