Thermo Scientific™ Decarboxylase Broth (Moeller) Lysine

Description

• Differentiate gram-negative enteric bacilli based on the enzymatic ability of an organism to decarboxylate lysine using Thermo Scientific™ Remel™ Decarboxylase Broth (Moeller) Lysine
• In 1955, Moeller developed the amino acid decarboxylase media to detect production of lysine and ornithine decarboxylase and arginine dihydrolase 1
• These media are used to differentiate bacterial groups within the Enterobacteriaceae 2
• Ewing, Davis, and Edwards reported Moeller's formulation to be more reliable for differentiating Klebsiella and Enterobacter as compared to other media tested 3
• The Moeller method is considered the standard or reference method for determining the decarboxylase reactions of the Enterobacteriaceae
• This medium helps differentiate gram negative bacilli by their ability to decarboxylate lysine
• The lysine decarboxylase test is used to differentiate between Salmonella and Shigella
• Recommended as the standard or reference method for determining decarboxylase reactions of Enterobacteriaceae
• Ready to Use: Convenience of prepared media
• Ease of Identification: Visual detection of color change
• This liquid medium is recommended for use in qualitative procedures
• This basal medium contains meat peptone and beef extract that provide the nitrogenous nutrients required for bacterial growth
• Dextrose acts as a source of energy and fermentable carbohydrate
• Bromcresol purple and cresol red are pH indicators
• Pyridoxal is an enzyme cofactor which enhances decarboxylase activity 4
• Lysine is an amino acid that is added to the basal medium to detect the production of enzymes which decarboxylase lysine
• When an organism ferments dextrose, acids are produced which lower the pH, resulting in a color change from purple to yellow
• If decarboxylation of lysine occurs in response to the acid pH, the alkaline end-product cadaverine (amine) is formed which results in the medium reverting to its original color (purple)
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.