Thermo Scientific™ HI Broth w/6.5% NaCl, BCP (5mL)

Description

• Differentiate Enterobacteriaceae from group D streptococci using Thermo Scientific™ Remel™ Heart Infusion Broth with 6.5% NaCl (with Brom Cresol Purple) (Salt Broth)
• Facklam demonstrated that the differentiation of enterococci and nonenterococcal group D streptococci was more accurate on using Heart Infusion Broth with 6.5% NaCl Modified with Indicator in combination with bile and esculin agar 1,2
• This media differentiates Enterobacteriaceae from group D streptococci
• Enterococci grow in the presence of 6.5% NaCl and utilize dextrose in the medium
• This medium contains beef heart infusion and casein peptone that supply nitrogenous compounds and amino acids required for the growth of streptococci
• Dextrose is an energy source
• Bacterial growth is enhanced by yeast extract which supplies essential B vitamins
• Ready to use – Continence of prepared media
• Selective media – Sodium chloride (6.5%) interferes with the membrane permeability and osmotic equilibrium and inhibits the growth of salt-intolerant bacteria Easy to interpret - Enterococci grow in the presence of 6.5% NaCl and utilize dextrose in the medium
• The metabolism of dextrose produces acid, which causes the indicator, brom cresol purple, to change from purple to yellow.