Determine fermentation reactions of bacteria with Thermo Scientific™ Remel™ Phenol Red Broth w/1% Dulcitol
Vera recommended using casein peptone in fermentation test media because of its high growth-promoting qualities 1
It was reported that peptone could be used with phenol red as the pH indicator in fermentation tests
Determine fermentation reactions of microorganisms using the liquid medium Phenol Red Broth w/1% Dulcitol
Phenol Red Broth w/1% Dulcitol is a complete basal medium prepared with phenol red as an indicator, which changes the medium to yellow when acid is produced
Carbohydrates may be added to the broth base in a final concentration of 1%
A Phenol Red Broth Base Control tube is used as a negative control for fermentation studies
A Durham tube may be inserted in a tube of Phenol Red Broth w/1% Dulcitol to allow detection of gas production
Can be used in fermentation studies of pure culture of many species of bacteria due to high growth promoting qualities of casein peptone
Recommended by AOAC International and the American Public Health Association (APHA) 2-5
Easy to interpret because of fermentation reaction and gas production
Yellow color is observed when there is acid production and red-orange color is observed when there is no fermentation
Bubbles are produced in the Durham tube when gas is produced and no bubbes is produced in the Durham tube when there is no gas production
Phenol Red Broth w/1% Dulcitol contains casein peptone that has high growth promoting qualities
Phenol red is the indicator which helps to determine the reaction by color change
This medium also contains Sodium Chloride, Phenol Red, Demineralized Water and Dulcitol
Sodium Chloride supplies essential electrolytes and maintains osmotic equilibrium of the medium
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.