Thermo Scientific™ Potassium Nitrate Assimilation Agar (Slant)

Description

• Perform the definative identification of yeast according to their ability to assimilate nitrogenous compounds with Thermo Scientific™ Remel™ Potassium Nitrate Assimilation Agar
• This medium was developed by Wickerham to determine assimilation of nitrogen by yeast 1
• Wickerham found that inclusion of vitamins was necessary for certain yeast 2
• Pincus et al
• modified the Wickerham formulation by adding higher concentrations of KNO 3 , yeast carbon base, pH indicator, and agar 3
• Identify yeast according to their ability to assimilate nitrogenous compounds using the solid medium Potassium Nitrate Assimilation Agar
• One criterion for the definitive identification of yeasts is the ability or inability to utilize an inorganic nitrogen source
• Potassium nitrate is added to test the ability of the isolate to assimilate nitrogen
• Bromthymol blue is the indicator used, which changes the medium from greenish yellow to blue-green or blue, indicating positive results
• Potassium Nitrate Assimilation Agar contains yeast carbon base, which provides amino acids, vitamins, trace elements and salts necessary to support growth of yeast
• Noble agar is a pure, low ash agar, which is a solidifying agent in this medium
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.