Thermo Scientific™ MacConkey Sorbitol Agar with Cefixime and Tellurite

Description

• Selectively detect sorbitol-negative Escherichia coli O157:H7 (VTEC) with Thermo Scientific™ Remel™ MacConkey Sorbitol Agar with Cefixime and Tellurite
• The medium is recommended by Food and Drug Administration Center for Food Safety and Applied Nutrition (FDA/CFSAN) and the American Public Health Association (APHA) for selective isolation of VTEC from food samples 6,7
• Verotoxin-producing Escherichia coli (VTEC), which includes serotype O157, is the fourth leading cause of diarrheal diseases in the United States 4
• This serotype of E
• coli produces a verotoxin and is indistinguishable on the routine isolation media from those of non-VTEC E.coli
• E
• coli O157 is recognized as a cause of haemorrhagic colitis, an illness characterized by bloody diarrhoea and severe abdominal pain, and haemolytic uraemic syndrome (HUS) 5 and as such, is a significant human pathogen
• Selectively detect sorbitol-negative Escherichia coli O157:H7 (VTEC) with MacConkey Sorbitol Agar with Cefixime and Tellurite
• Ease of differentiation: Due to the presence of D-sorbitol; sorbitol negative strains form colorless colonies while other E
• coli strains that ferment sorbitol and form pink colonies
• Selective: Bile salts and crystal violet are selective agents which inhibit gram-positive organisms and enterococci, respectively
• Cefixime and tellurite inhibit the growth of non-verotoxin producing E.coli and other non-sorbitol fermenting bacteria
• Recommended as a simple, inexpensive, rapid and reliable means of screening for E
• coli O157 Recommended by FDA, Center for Food Safety and Applied Nutrition (FDA/CFSAN) and theAPHA for selective isolation of VTEC from food samples 6,7
• In 1986, March and Ratnam introduced MacConkey Agar with sorbitol for the isolation of this serotype as unlike other serotypes VTEC does not ferment sorbitol 1
• In 1991, Chapman modified this medium by adding cefixime to specifically inhibit Proteus spp 2
• Zadik further modified it by adding potassium tellurite and found that it more effectively inhibited the growth of non-VTEC strains and increased isolation of E
• coli O157 3
• This medium contains peptones that provides nitrogenous compounds and amino acids essential for bacterial growth
• Bile salts and crystal violet are selective agents which inhibit gram-positive organisms
• Cefixime and tellurite inhibit the growth of non-verotoxin producing E.coli and other non-sorbitol fermenting bacteria
• Lactose is substituted with D-sorbitol
• Verotoxin- producing strains of E.coli ferment lactose but not sorbitol and form colorless colonies
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.