Thermo Scientific™ Pierce™ Protein Coated Plates

Description

• These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L)
• They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers
• The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA
• Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques
• Highlights • Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption • Orient antibodies for maximum antigen-binding capacity • Immobilize antibodies for plate assays without prior purification • Ensure minimal variation (<5% well-to-well) from consistent coating • Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer • Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG) Protein A/G Coated Plates • Fusion protein containing four antibody-binding sites from Protein A and two from Protein G • Binds to most IgG species because it combines the specificities of Protein A and Protein G • Binds to Fc region of antibodies, ensuring optimal orientation • Binds well using a wide range of pH Protein A Coated Plates • Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey • Binds strongly to mouse IgG2a, IgG2b and IgG3 • Binds to Fc region of antibodies for optimal orientation Protein G Coated Plates • Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat • Binds only to IgG—no cross-reactivity with other antibody classes • Binds to Fc region of antibodies for optimal orientation Protein L Coated Plates • Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD) • Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites • Binds to Fab fragments and ScFv • Does not bind bovine, goat or sheep immunoglobulin • Binds weakly to rabbit immunoglobulin • Pre-blocked to reduce nonspecific binding