PI15242

Thermo Scientific™ Pierce™ Nickel Coated Plates

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Pack of 5 PI15242-Pack-of-5 In Stock ₹ 32,427.24

PI15242 - Pack of 5

₹ 32,427.24

In Stock

Quantity

1

Base Price: ₹ 32,427.24

GST (18%): ₹ 5,836.903

Total Price: ₹ 38,264.143

Binding Property

Standard Capacity, Affinity

Description

White, 96-well

Packaging

5 Plates

Color

White

Material

Polystyrene

No. of Wells

96

Plate Blocking

Blocker™ BSA Buffer

Content And Storage

Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.

Detection Method

Chemiluminescence

Product Line

Pierce

Product Type

Microplate

Volume (Metric) Well

200 μL

Surface Treatment

Divalent Nickel, Metal Chelate

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Description

  • These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods
  • Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal
  • Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking
  • The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively
  • Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins
  • These white plates can be used with chemiluminescent detection methods
  • Features of Pierce Nickel Coated Plates include: • Ni 2+ activated surface enables metal-chelate binding of His-tagged proteins • Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene • Better binding for sensitive assays compared to other commercially available nickel-activated plates • Detection limit: 1 ng of polyhistidine fusion protein • Binding capacity: approx
  • 9 pmol His-tagged protein (27 kDa) per well • Activation level: 200 μL These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods
  • Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal
  • Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

Compare Similar Items

Show Difference

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Fischer Scientific

PI15242

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Binding Property:
Standard Capacity, Affinity

Description:
White, 96-well

Packaging:
5 Plates

Color:
White

Material:
Polystyrene

No. of Wells:
96

Plate Blocking:
Blocker™ BSA Buffer

Content And Storage:
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.

Detection Method:
Chemiluminescence

Product Line:
Pierce

Product Type:
Microplate

Volume (Metric) Well:
200 μL

Surface Treatment:
Divalent Nickel, Metal Chelate

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Fischer Scientific

PI15340

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Binding Property:
Standard Capacity, Affinity

Description:
Black, 96-well

Packaging:
5 Plates

Color:
Black

Material:
Polystyrene

No. of Wells:
96

Plate Blocking:
SuperBlock™ Buffer

Content And Storage:
Store at 4°C in unopened pouches. Once opened, store unused plates in a resealable bag with desiccant at 4°C.

Detection Method:
Fluorescence

Product Line:
Pierce

Product Type:
Microplate

Volume (Metric) Well:
__

Surface Treatment:
Glutathione

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Fischer Scientific

PI15342

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Binding Property:
Standard Capacity, Affinity

Description:
Black, 96-well

Packaging:
5 Plates

Color:
Black

Material:
Polystyrene

No. of Wells:
96

Plate Blocking:
Blocker™ BSA Buffer

Content And Storage:
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.

Detection Method:
Fluorescence

Product Line:
Pierce

Product Type:
Microplate

Volume (Metric) Well:
200 μL

Surface Treatment:
Divalent Nickel, Metal Chelate

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Fischer Scientific

PI15400

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Binding Property:
Standard Capacity, Affinity

Description:
Clear, 384-well

Packaging:
__

Color:
Clear

Material:
Polystyrene

No. of Wells:
384

Plate Blocking:
SuperBlock™ Buffer

Content And Storage:
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. Plates are shipped at ambient temperature.

Detection Method:
Colorimetric

Product Line:
NeutrAvidin Pierce

Product Type:
Microplate

Volume (Metric) Well:
__

Surface Treatment:
NeutrAvidin Protein