Thermo Scientific™ Pierce™ Nickel Coated Plates

Description

• These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods
• Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal
• Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking
• The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively
• Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins
• These white plates can be used with chemiluminescent detection methods
• Features of Pierce Nickel Coated Plates include: • Ni 2+ activated surface enables metal-chelate binding of His-tagged proteins • Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene • Better binding for sensitive assays compared to other commercially available nickel-activated plates • Detection limit: 1 ng of polyhistidine fusion protein • Binding capacity: approx
• 9 pmol His-tagged protein (27 kDa) per well • Activation level: 200 μL These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods
• Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal
• Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.