69041
B834(DE3) Competent Cells - Novagen
Escherichia coli, rod shaped
Manufacturer: Sigma Aldrich
Synonym(S): Competent cells
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.4 ML | 69041-0.4-ML | In Stock | ₹ 14,380.00 |
| 1 ML | 69041-1-ML | In Stock | ₹ 32,920.01 |
69041 - 0.4 ML
In Stock
Quantity
1
Base Price: ₹ 14,380.00
GST (18%): ₹ 2,588.40
Total Price: ₹ 16,968.40
product name
B834(DE3) Competent Cells - Novagen, B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.
biological source
Escherichia coli
manufacturer/tradename
Novagen®
storage condition
OK to freezeavoid repeated freeze/thaw cycles
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
transformation efficiency: >2×106 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Related Products
Description
- General description: B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
- Components: 0.4 ml1 mlComponent•2 × 0.2 ml5 × 0.2 mlB834(DE3)Competent Cells•2 × 2 ml4 × 2 mlSOC Medium•2 ng2 ngTest Plasmid
- Warning: Toxicity: Multiple Toxicity Values, refer to MSDS (O)
- Other Notes: 1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
- Legal Information: NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
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product name: B834(DE3) Competent Cells - Novagen, B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.
biological source: Escherichia coli
manufacturer/tradename: Novagen®
storage condition: OK to freezeavoid repeated freeze/thaw cycles
growth mode: adherent or suspension
morphology: rod shaped
technique(s): microbiological culture: suitable
cell transformation: transformation efficiency: >2×106 cfu/μg
shipped in: dry ice
storage temp.: −70°C
product name:
B834(DE3) Competent Cells - Novagen, B834 is the parental strain for BL21. These hosts are methionine auxotrophs and allow high specific activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography.
biological source:
Escherichia coli
manufacturer/tradename:
Novagen®
storage condition:
OK to freezeavoid repeated freeze/thaw cycles
growth mode:
adherent or suspension
morphology:
rod shaped
technique(s):
microbiological culture: suitable
cell transformation:
transformation efficiency: >2×106 cfu/μg
shipped in:
dry ice
storage temp.:
−70°C
product name: __
biological source: __
manufacturer/tradename: Arxada AG
storage condition: __
growth mode: __
morphology: __
technique(s): __
cell transformation: __
shipped in: __
storage temp.: 2-8°C
product name:
__
biological source:
__
manufacturer/tradename:
Arxada AG
storage condition:
__
growth mode:
__
morphology:
__
technique(s):
__
cell transformation:
__
shipped in:
__
storage temp.:
2-8°C
product name: B834(DE3)pLysS Competent Cells - Novagen, B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression.
biological source: Escherichia coli
manufacturer/tradename: Novagen®
storage condition: OK to freezeavoid repeated freeze/thaw cycles
growth mode: adherent or suspension
morphology: rod shaped
technique(s): microbiological culture: suitable
cell transformation: transformation efficiency: >2×106 cfu/μg
shipped in: dry ice
storage temp.: __
product name:
B834(DE3)pLysS Competent Cells - Novagen, B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression.
biological source:
Escherichia coli
manufacturer/tradename:
Novagen®
storage condition:
OK to freezeavoid repeated freeze/thaw cycles
growth mode:
adherent or suspension
morphology:
rod shaped
technique(s):
microbiological culture: suitable
cell transformation:
transformation efficiency: >2×106 cfu/μg
shipped in:
dry ice
storage temp.:
__
product name: __
biological source: __
manufacturer/tradename: __
storage condition: __
growth mode: __
morphology: __
technique(s): __
cell transformation: __
shipped in: __
storage temp.: −20°C
product name:
__
biological source:
__
manufacturer/tradename:
__
storage condition:
__
growth mode:
__
morphology:
__
technique(s):
__
cell transformation:
__
shipped in:
__
storage temp.:
−20°C