B834(DE3)pLysS Competent Cells - Novagen

Description

• General description: B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2).DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
• Features and Benefits: Allows for 35S-methionine or selenomethionine labeling for crystallography studiesDeficient in lon and ompT proteasesGuaranteed transformation efficiencies of >2x106 cfu/μgProvided in a 0.2 mL volume; each vial can be used for 10 x 20μL transformationsPrepared using an optimized chemical method
• Components: 0.4 ml1 mlComponent•2 × 0.2 ml5 × 0.2 mlB834(DE3)pLysS Competent Cells•2 × 2 ml4 × 2 mlSOC Medium•2 ng2 ngTest Plasmid
• Warning: Toxicity: Multiple Toxicity Values, refer to MSDS (O)
• Other Notes: 1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133.2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991.
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