B2904
Bluo-Gal
reagent for selection of recombinant bacterial clones
Manufacturer: Sigma Aldrich
CAS Number: 97753-82-7
Synonym(S): 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 MG | B2904-100-MG | In Stock | ₹ 18,175.18 |
| 500 MG | B2904-500-MG | In Stock | ₹ 60,717.43 |
B2904 - 100 MG
In Stock
Quantity
1
Base Price: ₹ 18,175.18
GST (18%): ₹ 3,271.532
Total Price: ₹ 21,446.712
grade
for molecular biology
Quality Level
200
sterility
non-sterile
Assay
≥98% (HPLC)
form
powder
technique(s)
nucleic acid detection: suitable
solubility
DMF: soluble
suitability
suitable for substrate for β-galactosidase
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
 | Sigma Aldrich Fine Chemicals Biosciences Bluo-Gal | 97753-82-7 | MFCD00063691 | 100mg | Sigma Aldrich Fine Chemicals Biosciences | ₹ 18,656.36 | |
 | eMolecules Medchem Express / 5-Bromo-3-indolyl -D-galactopyranoside / 100mg / 742367634 / HY-137276 / / 97753-82-7 / MFCD00063691 / 374.187 / C14H16BrNO6 | eMolecules | ₹ 11,964.71 | |
 | 5-Bromo-3-indolyl β-D-galactopyranoside | ChemScene | ₹ 2,395.68 - ₹ 16,256.40 | |
 | 97753-82-7 | 5-Bromo-3-indolyl-beta-d-galactopyranoside | A2B Chem | ₹ 855.60 - ₹ 28,234.80 |
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Description
- General description: Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
- Application: Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis,[1] Dictyostelium samples[2] and in the staining of vena cava of adult mice.[3]
- Principle: When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Compare Similar Items
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grade: for molecular biology
Quality Level: 200
sterility: non-sterile
Assay: ≥98% (HPLC)
form: powder
technique(s): nucleic acid detection: suitable
solubility: DMF: soluble
suitability: suitable for substrate for β-galactosidase
storage temp.: −20°C
SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
grade:
for molecular biology
Quality Level:
200
sterility:
non-sterile
Assay:
≥98% (HPLC)
form:
powder
technique(s):
nucleic acid detection: suitable
solubility:
DMF: soluble
suitability:
suitable for substrate for β-galactosidase
storage temp.:
−20°C
SMILES string:
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
grade: __
Quality Level: 200
sterility: __
Assay: ≥97%
form: solid
technique(s): __
solubility: __
suitability: __
storage temp.: __
SMILES string: [Na+].[O-]S(=O)(=O)CCCBr
grade:
__
Quality Level:
200
sterility:
__
Assay:
≥97%
form:
solid
technique(s):
__
solubility:
__
suitability:
__
storage temp.:
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SMILES string:
[Na+].[O-]S(=O)(=O)CCCBr
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