B6650

X-GlcA

reagent for selection of recombinant bacterial clones

Manufacturer: Sigma Aldrich

CAS Number: 114162-64-0

Synonym(S): 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, X-GlcA, X-glucuronide CHA salt

Select a Size

Pack Size SKU Availability Price
10 MG B6650-10-MG In Stock ₹ 18,240.13
25 MG B6650-25-MG In Stock ₹ 27,625.40
100 MG B6650-100-MG In Stock ₹ 91,904.25

B6650 - 10 MG

₹ 18,240.13

In Stock

Quantity

1

Base Price: ₹ 18,240.13

GST (18%): ₹ 3,283.223

Total Price: ₹ 21,523.353

grade

Molecular Biology Reagentfor molecular biology

Quality Level

200

sterility

non-sterile

Assay

≥98% (TLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

Other Options

Image Product Name Manufacturer Price Range
50-491-589
Chem-Impex International, Inc. 5-Bromo-4-chloro-3-indolyl-b-D-glucuronide cyclohexylammonium salt | 114162-64-0 | MFCD00058543 | 100MG
Chem-Impex International, Inc. ₹ 3,736.41
50-245-5078
eMolecules​ Medchem Express / X-Gluc (cyclohexanamine) / 5mg / 783660583 / HY-15935B / / 114162-64-0 / MFCD00058543 / 521.790 / C20H26BrClN2O7
eMolecules​ ₹ 8,816.10
AR000BE2
β-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1)
Aaron Chemicals LLC ₹ 1,967.88 - ₹ 20,791.08
CS-0019449
X-Gluc cyclohexanamine
ChemScene ₹ 4,705.80 - ₹ 37,560.84
AA13662
114162-64-0 | X-Beta-d-glcua dcha h2o
A2B Chem ₹ 2,139.00 - ₹ 56,640.72

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Description

  • General description: X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
  • Application: Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.X-GlcA has been used in histochemical staining of root sections for light microscopic observation.[1]
  • Principle: When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.

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Molecular Biology Reagentfor molecular biology

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200

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Assay:
≥98% (TLC)

form:
powder

technique(s):
nucleic acid detection: suitable

solubility:
DMF: soluble

suitability:
suitable for β-galactosidase test

storage temp.:
−20°C

SMILES string:
NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

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BrC1c2ccccc2-c3ccccc13

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SMILES string:
Fc1cccc(Br)c1