L2897

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

Manufacturer: Sigma Aldrich

Synonym(S): Lennox broth, Luria Bertani Agar

Select a Size

Pack Size SKU Availability Price
250 G L2897-250-G In Stock ₹ 8,486.80
1 KG L2897-1-KG In Stock ₹ 24,691.83

L2897 - 250 G

₹ 8,486.80

In Stock

Quantity

1

Base Price: ₹ 8,486.80

GST (18%): ₹ 1,527.624

Total Price: ₹ 10,014.424

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L NaCl, 5 g/L Tryptone, 10 g/L Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

food and beveragesmicrobiology

storage temp.

room temp

suitability

nonselective for Escherichia colinonselective for coliforms

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Description

  • General description: Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.
  • Application: LB Broth with agar (Lennox) has been used for:the transformation of competent E. coli cells[1][1] to cultivate Ecc-15 bacteria[2] embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs) transmission electron microscopy (TEM)[3][4] and immunohistochemistry (IHC)[3]It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
  • Features and Benefits: Lennox LB powder with agar provides:A budget-friendly alternative to pre-poured platesEasy scale-up using larger package sizesStandard formulation
  • Preparation Note: 1. Suspend 35 g in 1 L of distilled water.2. Heat to boiling while stirring to dissolve all ingredients completely.3. Autoclave for 15 minutes at 121°C.To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.
  • Legal Information: Zeocin is a registered trademark of Cayla Sarl

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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microbiological culture: suitable

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