LB Broth with agar (Miller)

Description

• General description: Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
• Application: LB Broth with agar (Miller) has been used for the generation of single colony for Pseudomonas putida KT2440,[1] plating E. coli[2] and for the generation of pure colonies of C14 and MC8M6 of bacterial strains.[3]
• Features and Benefits: Miller′s LB powder with agar provides:Easy scale-up using larger package sizesA budget-friendly alternative to pre-poured platesStandard formulation
• Preparation Note: 1. Suspend 40 g in 1L of distilled water.2. Heat to boiling while stirring to dissolve.3. Autoclave for 15 minutes at 121°C.4. Cool to 50°C prior to dispensing into sterile petri dishes.To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.
• Reconstitution: Add 40g powder to 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).