CMC0001
SIG10 Chemically Competent Cells
Escherichia coli, rod shaped
Manufacturer: Sigma Aldrich
Synonym(S): Chemically competent E. coli, Competent bacterial cells
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 4 X 40 μL | CMC0001-4-X-40-μL | In Stock | ₹ 8,524.80 |
| 40 X 40 μL | CMC0001-40-X-40-μL | In Stock | ₹ 72,649.50 |
CMC0001 - 4 X 40 μL
In Stock
Quantity
1
Base Price: ₹ 8,524.80
GST (18%): ₹ 1,534.464
Total Price: ₹ 10,059.264
product name
SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production
biological source
Escherichia coli
grade
for molecular biology
form
buffered aqueous solution
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competenttransformation efficiency: ≥1 × 109 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Related Products
Description
- General description: The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications. They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively. The cells have a transformation efficiency of >1 x 109 cfu/μg The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg.GenotypeF- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- Application: Suitable for bacterial transformations to recover high quality plasmid DNA
- Features and Benefits: share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.ensures recovery of stable and high quality plasmid DNA.high transformation efficiencyprovide the performance researchers need with ease of use.provide solutions for a wide range of applications at economical prices.Blue - white screening
- Components: SIG10 chemically competent cellspUC 19 transformation control DNA at 10 pg/μLrecovery medium for cloning
- Principle: The SIG10 cells contains the inactive mcr and mrr alleles, allowing methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements. This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.
- Legal Information: DH5α is a trademark of Invitrogen Corp.
SAFETY INFORMATION
WGK
WGK 3
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product name: SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production
biological source: Escherichia coli
grade: for molecular biology
form: buffered aqueous solution
growth mode: adherent or suspension
morphology: rod shaped
technique(s): microbiological culture: suitable
cell transformation: competent cell type: chemically competenttransformation efficiency: ≥1 × 109 cfu/μg
shipped in: dry ice
storage temp.: −70°C
product name:
SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production
biological source:
Escherichia coli
grade:
for molecular biology
form:
buffered aqueous solution
growth mode:
adherent or suspension
morphology:
rod shaped
technique(s):
microbiological culture: suitable
cell transformation:
competent cell type: chemically competenttransformation efficiency: ≥1 × 109 cfu/μg
shipped in:
dry ice
storage temp.:
−70°C
product name: SIG10 MAX Electrocompetent Cells, for general cloning & library production
biological source: Escherichia coli
grade: for molecular biology
form: buffered aqueous suspension
growth mode: adherent or suspension
morphology: rod shaped
technique(s): microbiological culture: suitable
cell transformation: competent cell type: electrocompetenttransformation efficiency: ≥2 x 1010 cfu/μg
shipped in: dry ice
storage temp.: −70°C
product name:
SIG10 MAX Electrocompetent Cells, for general cloning & library production
biological source:
Escherichia coli
grade:
for molecular biology
form:
buffered aqueous suspension
growth mode:
adherent or suspension
morphology:
rod shaped
technique(s):
microbiological culture: suitable
cell transformation:
competent cell type: electrocompetenttransformation efficiency: ≥2 x 1010 cfu/μg
shipped in:
dry ice
storage temp.:
−70°C
product name: SIG10 5a Chemically Competent Cells, for DNA plasmid production
biological source: Escherichia coli
grade: for molecular biology
form: buffered aqueous solution
growth mode: adherent or suspension
morphology: rod shaped
technique(s): microbiological culture: suitable
cell transformation: competent cell type: chemically competenttransformation efficiency: ≥1 x 108 cfu/μg
shipped in: dry ice
storage temp.: −70°C
product name:
SIG10 5a Chemically Competent Cells, for DNA plasmid production
biological source:
Escherichia coli
grade:
for molecular biology
form:
buffered aqueous solution
growth mode:
adherent or suspension
morphology:
rod shaped
technique(s):
microbiological culture: suitable
cell transformation:
competent cell type: chemically competenttransformation efficiency: ≥1 x 108 cfu/μg
shipped in:
dry ice
storage temp.:
−70°C
product name: BL21(DE3) Chemically Competent Cells, for protein expression
biological source: Escherichia coli
grade: for molecular biology
form: __
growth mode: adherent or suspension
morphology: rod shaped
technique(s): microbiological culture: suitable
cell transformation: competent cell type: chemically competenttransformation efficiency: ≥1 x 107 cfu/μg
shipped in: dry ice
storage temp.: −70°C
product name:
BL21(DE3) Chemically Competent Cells, for protein expression
biological source:
Escherichia coli
grade:
for molecular biology
form:
__
growth mode:
adherent or suspension
morphology:
rod shaped
technique(s):
microbiological culture: suitable
cell transformation:
competent cell type: chemically competenttransformation efficiency: ≥1 x 107 cfu/μg
shipped in:
dry ice
storage temp.:
−70°C