OverExpress™ C43(DE3) Chemically Competent Cells

Description

• General description: OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.Genotype F – ompT hsdSB (rB- mB-) gal dcm (DE3)
• Application: OverExpress™ C43(DE3) Chemically Competent Cells have been used for transforming the toll/interleukin receptor (TIR) domain constructs of sterile alpha and TIR motif–containing protein 1 (SARM1).[1]
• Features and Benefits: Express genes cloned into any T7 vector with these BL21(DE3) derivativesEffective in expressing toxic & membrane proteinsCited in over 350 research articles
• Components: OverExpress C43(DE3) chemically competent cellspUC 19 transformation control DNArecovery medium for expression
• Legal Information: OverExpress is a trademark of Imaxio, S.A.