L3037
Escort™ III Transfection Reagent
Lipid reagent for transfecting sensitive and primary cells
Manufacturer: Sigma Aldrich
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 ML | L3037-1-ML | In Stock | ₹ 22,311.00 |
L3037 - 1 ML
In Stock
Quantity
1
Base Price: ₹ 22,311.00
GST (18%): ₹ 4,015.98
Total Price: ₹ 26,326.98
grade
for molecular biology
Quality Level
200
form
liquid (aqueous solution)
usage
mL sufficient for 250-1000 transfections
concentration
1 mg/mL
technique(s)
transfection: suitable
storage temp.
2-8°C
Related Products
Description
- General description: Escort™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.
- Application: Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ III: A549C2C12 myotubesCardiomyocytes (rat)COS-7Fibroblasts (rat)Germ cells (male rat)Hepatocytes (rat and hamster)HepG2HeLaJurkatKeratinocytes (human)Myoblasts (mouse and quail)Myocytes (mouse)NIH3T3PC-12Retinal Neurons (rat)Tracheobronchial cells (sheep)
- Features and Benefits: Suitable for stable and transient transfection Optimized for a wide variety of primary cellsLow toxicityCompatible with both serum and serum-free transfection protocols Ideal for PC-12 cells
- Components: Escort™ III formulation:1 mg/mL total lipid in waterNote the identity of the lipids used in Escort™ III is confidential.
- Caution: Do not freeze.
- Principle: A stable complex is formed when Escort™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.
- Legal Information: Escort is a trademark of Sigma-Aldrich Co. LLC
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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grade: for molecular biology
Quality Level: 200
form: liquid (aqueous solution)
usage: mL sufficient for 250-1000 transfections
concentration: 1 mg/mL
technique(s): transfection: suitable
storage temp.: 2-8°C
grade:
for molecular biology
Quality Level:
200
form:
liquid (aqueous solution)
usage:
mL sufficient for 250-1000 transfections
concentration:
1 mg/mL
technique(s):
transfection: suitable
storage temp.:
2-8°C
grade: __
Quality Level: 400
form: __
usage: __
concentration: __
technique(s): __
storage temp.: __
grade:
__
Quality Level:
400
form:
__
usage:
__
concentration:
__
technique(s):
__
storage temp.:
__
grade: __
Quality Level: 200
form: lyophilized powder
usage: __
concentration: __
technique(s): __
storage temp.: 2-8°C
grade:
__
Quality Level:
200
form:
lyophilized powder
usage:
__
concentration:
__
technique(s):
__
storage temp.:
2-8°C
grade: __
Quality Level: 200
form: lyophilized powder
usage: __
concentration: __
technique(s): __
storage temp.: 2-8°C
grade:
__
Quality Level:
200
form:
lyophilized powder
usage:
__
concentration:
__
technique(s):
__
storage temp.:
2-8°C