Yeast Transformation Kit

Description

• General description: Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.
• Application: Suitable for transformation of any strain of yeast.[1][2][3] Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.
• Features and Benefits: Easy and ready-to-useRequires as little as 10 ng of plasmid DNAFlexibility for any strain of yeastSufficient for over 100 standard transformations
• Components: The Yeast Transformation Kit contains:Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTAPlate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTADeoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 mlControl Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μgYeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g
• Principle: Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).