CAPHOS
Calcium Phosphate Transfection Kit
Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
Manufacturer: Sigma Aldrich
Synonym(S): Gene delivery
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 KIT | CAPHOS-1-KIT | In Stock | ₹ 46,103.68 |
CAPHOS - 1 KIT
In Stock
Quantity
1
Base Price: ₹ 46,103.68
GST (18%): ₹ 8,298.662
Total Price: ₹ 54,402.342
grade
for molecular biology
Quality Level
200
form
solution
usage
kit sufficient for 160 transfections (6 cm dishes) kit sufficient for 400 transfections (3.5 cm dishes) kit sufficient for 80 transfections (10 cm dishes)
technique(s)
transfection: suitable
shipped in
dry ice
storage temp.
−20°C
Related Products
Description
- General description: Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.
- Application: Calcium Phosphate Transfection Kit has been used:to enable transfection[1]to transfect Hek293T cells[2]to transfect H29D cells[3]
- Features and Benefits: Suitable for transient and stable transfectionReproducible for a wide range of cell typesWidely referencedInexpensive
- Components: The Calcium Phosphate Transfection Kit contains:5 ml 2.5M CaCl2 (C2052)25 ml 2x HEPES Buffered Saline (H1012)25 ml molecular biology grade water (W4502)
- Principle: The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.
- Other Notes: The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.
SAFETY INFORMATION
Pictograms
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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grade: for molecular biology
Quality Level: 200
form: solution
usage: kit sufficient for 160 transfections (6 cm dishes) kit sufficient for 400 transfections (3.5 cm dishes) kit sufficient for 80 transfections (10 cm dishes)
technique(s): transfection: suitable
shipped in: dry ice
storage temp.: −20°C
grade:
for molecular biology
Quality Level:
200
form:
solution
usage:
kit sufficient for 160 transfections (6 cm dishes) kit sufficient for 400 transfections (3.5 cm dishes) kit sufficient for 80 transfections (10 cm dishes)
technique(s):
transfection: suitable
shipped in:
dry ice
storage temp.:
−20°C
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storage temp.: 2-8°C
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