Yeast Nitrogen Base With Amino Acids

Description

• General description: Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.
• Application: Yeast Nitrogen Base With Amino Acids has been used:in the ZYP-31 media preparation for E.coli[1]in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source[2]to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard[3]in the preparation of imaging medium[4]Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.
• Components: Component, amount (μg/L unless indicated)Nitrogen Sources:Ammonium sulfate, 5.0 g/LAmino Acids:L-histidine, 10 mg/LDL-methionine, 20 mg/LDL-tryptophan, 20 mg/LVitamins:Biotin, 2.0Calcium pantothenate, 400Folic acid, 2.0Inositol, 2.0 mg/LNicotinic acid, 400p-Aminobenzoic acid, 200Pyridoxine HCl, 400Riboflavin, 200Thiamine HCL, 400Trace Elements:Boric acidCopper sulfatePotassium iodideFerric chlorideMagnesium sulfateSodium molybdateZinc sulfateSalts:Potassium phosphate monobasic, 1.0 g/LMagnesium sulfate, 0.5 g/LSodium chloride, 0.1 g/LCalcium chloride, 0.1 g/L
• Reconstitution: Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.