Cas9 Hygromycin Lentiviral Particles

Description

• General description: Ready-to-use Cas9-Hygro lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-Hygro lentiviral particles efficiently and stably integrate Cas9 and a hygromycin resistance cassette linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-Hygro) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. To order gRNA in any format click here
• Application: Functional Genomics/Target ValidationCreation of gene knockouts in multiple cell linesComplete knockout of genes not amenable to RNAiCreation of cell lines stably expressing Cas9
• Features and Benefits: Highly specific and highly activeSequence verifiedReady to infect/transduce
• Principle: CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
• Unit Definition: VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.
• Legal Information: CRISPR Label LicenseLentiviral, WPRE, and Evrogen Licenses