SHC216
MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA
Targets no known genes from any species
Manufacturer: Sigma Aldrich
Synonym(S): MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 EA | SHC216-1-EA | In Stock | ₹ 41,232.43 |
SHC216 - 1 EA
In Stock
Quantity
1
Base Price: ₹ 41,232.43
GST (18%): ₹ 7,421.837
Total Price: ₹ 48,654.267
Quality Level
200
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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Description
- General description: The MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones.[1][2] This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
- Application: To see more application data, protocols, vector maps visit sigma.com/shrna.
- Legal Information: Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
SAFETY INFORMATION
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Quality Level: 200
product line: MISSION®
concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
product line:
MISSION®
concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: 100200
product line: MISSION®
concentration: ≥1x106 VP/ml (via p24 assay)
shipped in: dry ice
storage temp.: −70°C
Quality Level:
100200
product line:
MISSION®
concentration:
≥1x106 VP/ml (via p24 assay)
shipped in:
dry ice
storage temp.:
−70°C
Quality Level: 200
product line: MISSION®
concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
product line:
MISSION®
concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: 100200
product line: MISSION®
concentration: ≥1x106 VP/ml (via p24 assay)
shipped in: dry ice
storage temp.: −70°C
Quality Level:
100200
product line:
MISSION®
concentration:
≥1x106 VP/ml (via p24 assay)
shipped in:
dry ice
storage temp.:
−70°C