MISSION® LentiPlex® Human Pooled shRNA Library

Description

• General description: MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma¢s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation.Please visit sigma.com/lentiplex to learn more and see example data and uses.
• Application: MISSION® LentiPlex® Human Pooled shRNA Library has been used to transfect human colon adenocarcinoma cell line HT29 for shRNA screening and bioinformatics analysis.[1]
• Other Notes: Deconvolution of shRNA PoolsSigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample Comprehensive, reproducible results from pooled shRNA screens Statistically robust and information-rich data Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi
• Legal Information: Lentiviral License Agreement