DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation

Description

• General description: The DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of single digoxigenin-labeled dideoxyuridine triphosphates (ddUTP) to the 3′-OH end of oligonucleotides. Thus, helps in labeling oligonucleotides.
• Application: DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation is for 3′-end labeling of oligonucleotides from 14 to 100 nucleotides in length with DIG-11-ddUTP and recombinant terminal transferase.DIG-labeled oligonucleotides has been used in a variety of hybridization techniques: dot/slot blotscolony/ plaque hybridizationsSouthern blots/ northern blots[1]in situ hybridizations[2]
• Features and Benefits: Fast hybridization kinetics, due to the small size of oligonucleotidesSingle-stranded probes, no renaturation during hybridizationSequence can be designed according to the experimentSpecially suited for in situ hybridization; due to their small size, the oligonucleotides readily diffuse into fixed tissues and cells
• Packaging: 1 kit containing 9 components
• Quality: Function tested in a dot blot.
• Principle: One DIG-ddUTP molecule is added to the 3′-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction.
• Preparation Note: Activator: sodium sulfate, TrisWorking concentration: Oligonucleotides: 100 pmolUp to 100 pmol (1 μg of a 30-mer) oligonucleotide can be labeled in a single standard labeling reaction.
• Storage and Stability: Store at -15–-25 °C. (unopened kit)
• Other Notes: For life science research only. Not for use in diagnostic procedures.