DIG DNA Labeling and Detection Kit

Description

• General description: DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers.Contents:Unlabeled Control DNA 1, 100 μg/mlUnlabeled Control DNA 2, 200 μg/mlDNA Dilution BufferDIG-labeled Control DNA, 5.2 μg/ml10x Hexanucleotide Mix10x dNTP Labeling MixtureKlenow Enzyme, Labeling grade, 2 U/μlAnti-digoxigenin-AP-conjugate, 750 U/mlNBT/BCIP Concentrated Stock SolutionBlocking Reagent
• Specificity: Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.
• Application: DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques: Southern blots[1][1]Northern blots[2]Dot blotsColony and plaque screeningDIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light blue precipitate.
• Packaging: 1 kit containing 10 components.
• Preparation Note: Working concentration: Dilution of Antibody 1:5,000
• Other Notes: For life science research only. Not for use in diagnostic procedures.